Daystar University was first housed at the Nairobi Campus. This campus is located approximately two kilometres from the City Center, at the corner of Ngong Road and Valley Roads. It is a few minutes’ walk from Hurlingham shopping Center, the Kenyatta National Hospital and Nairobi Hospital. It also neighbours Nairobi Baptist Church and is easily accessible by public and private means. The campus hosts the diploma programmes, day and evening undergraduate programmes, Master’s programmes in Communication, Counseling Psychology, Theology, Economics, Monitoring and Evaluation, Child Development and Business, and PhD in Communication and Clinical Psychology. 

Daystar University launched a new modern and well-equipped fitness facility in March 2021. The Jack and Camie Eugster Fitness Center was commissioned by the Daystar Co- founder Dr. Donald Smith and The Vice Chancellor, Prof. Laban Ayiro at the Athi River Campus. The commissioning of the gym was part of a joint launch of the redefined University’s Strategic Plan. The process of the strategic plan was a result of self-examination and engagement with stakeholders to challenge the status quo to accommodate the new way of life brought about the Covid-19 pandemic.